https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34067 Wed 06 Feb 2019 09:51:04 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:41831 -1 and 400 mg kg^-1), F1 and F2 transformed to F4 and F5. Meanwhile, soil moisture had a significant influence on soil ALP activity. Soil ALP activity under 110% WHC was smaller than the activity of soil ALP under 35% WHC. The variation partitioning analysis (VPA) showed that soil moisture contributed 63.19% for this effect on soil ALP activity and was identified as the dominant factor. The value of ED 10 indicated that the As toxicity under 35% WHC was greater than that under 110% WHC. This could be due to that the excessive moisture content depressed soil ALP activity. Therefore, it is important to consider soil moisture content while assessing the As toxicity to soil ALP.]]> Fri 26 Aug 2022 11:21:16 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32983 max) and Michaelis constant (K_m) in unpolluted soils were 0.012-0.267 mM h^-1 and 1.34-3.79 mM respectively. The competitive inhibition constant (K_ic) was 0.17-0.70 mM, which was lower than K_m, suggesting higher enzyme affinity for As than for substrate. The ecological doses, ED₁₀ and ED₅₀ (concentration of As that results in 10% and 50% inhibition on enzyme parameter) for inhibition of catalytic efficiency (V_max/K_m) were lower than those for inhibition of enzyme activity at different substrate concentrations. This suggests that the integrated kinetic parameter, catalytic efficiency is substrate concentration independent and more sensitive to As than ALP activity. Thus, catalytic efficiency was proposed as a more reliable indicator than ALP activity for risk assessment of As pollution.]]> Fri 17 Aug 2018 15:44:20 AEST ]]>